Anticorps Recombinant de lapin anti-Collagen Type I

Collagen Type I Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240665D2

N° de cat : 83752-3-PBS

Synonymes

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

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Galerie de données de validation

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  WB analysis of rat skin using 83752-3-RR (same clone as 83752-3-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

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  WB analysis using 83752-3-RR (same clone as 83752-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83752-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00716-3

  Sandwich ELISA standard curve of MP00716-3, Human Collagen Type I Recombinant Matched Antibody Pair - PBS only. 83752-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6281. 83752-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00716-3

  Serum of sixteen individual healthy human donors was measured. The Collagen Type I concentration of detected samples was determined to be 2.67 ng/mL with a range of ND-15.45 ng/mL.

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  Affinity and Kinetic Characterization of 83752-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83752-3-PBS against Human Collagen Type I was performed. The affinity constant is 47.7 pM.

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  Affinity and Kinetic Characterization of 83752-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

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Informations sur le produit

83752-3-PBS cible Collagen Type I dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Collagen Type I Protéine recombinante Ag6281
• Nom complet: collagen, type I, alpha 2
• Masse moléculaire calculée: 1366 aa, 130 kDa
• Poids moléculaire observé: 100-160 kDa
• Numéro d’acquisition GenBank: BC054498
• Symbole du gène: COL1A2
• Identification du gène (NCBI): 1278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

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