Anticorps Recombinant de lapin anti-Collagen Type I

Collagen Type I Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240665D2

N° de cat : 83752-3-RR

Synonymes

COL1, COL1A2, 240665D2, Alpha-2 type I collagen, col I

---

Galerie de données de validation

• 

  WB analysis of rat skin using 83752-3-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83752-3-RR (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83752-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83752-3-RR (COL1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83752-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83752-3-RR against Human Collagen Type I were performed. The affinity constant is 0.777 nM.

---

Applications testées

• Résultats positifs en WB: MG-63 cells, tissu cutané de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83752-3-RR cible Collagen Type I dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Collagen Type I Protéine recombinante Ag6281
• Nom complet: collagen, type I, alpha 2
• Masse moléculaire calculée: 1366 aa, 130 kDa
• Poids moléculaire observé: 100-160 kDa
• Numéro d’acquisition GenBank: BC054498
• Symbole du gène: COL1A2
• Identification du gène (NCBI): 1278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

{{ptg:RelatedPrimaryAntibodies}}