Anticorps Recombinant de lapin anti-Collagen Type I

Collagen Type I Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240665F8

N° de cat : 83752-5-PBS

Synonymes

COL1A2, COL1, Collagen alpha 2(I) chain, col I, Alpha-2 type I collagen

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Galerie de données de validation

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  IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 83752-5-RR (same clone as 83752-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83752-5-RR (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using COL1A2 antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  IF Staining of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HSC-T6 cells using Collagen Type I antibody (83752-5-RR, Clone: 240665F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  FC experiment of HSC-T6 using 83752-5-RR (same clone as 83752-5-PBS)

  1x10^6 HSC-T6 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  FC experiment of SW480 using 83752-5-RR (same clone as 83752-5-PBS)

  1x10^6 SW480 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant antibody (83752-5-RR, Clone:240665F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83752-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83752-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83752-5-RR against Human COL1A2 were performed. The affinity constant is 6.77 nM.

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Informations sur le produit

83752-5-PBS cible Collagen Type I dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Collagen Type I Protéine recombinante Ag6281
• Nom complet: collagen, type I, alpha 2
• Masse moléculaire calculée: 1366 aa, 130 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC054498
• Symbole du gène: COL1A2
• Identification du gène (NCBI): 1278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

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