Anticorps Monoclonal anti-Collagen Type III

Collagen Type III Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B1F3

N° de cat : 68320-1-PBS

Synonymes

COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain

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Galerie de données de validation

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  WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

  human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

  rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COL3A1 antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68320-1-PBS cible Collagen Type III dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Collagen Type III Protéine recombinante Ag4229
• Nom complet: collagen, type III, alpha 1
• Masse moléculaire calculée: 1466 aa, 139 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC028178
• Symbole du gène: COL3A1
• Identification du gène (NCBI): 1281
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).

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