Anticorps Recombinant de lapin anti-Collagen Type III (N-terminal)

Collagen Type III (N-terminal) Recombinant Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2O1

N° de cat : 80009-1-RR

Synonymes

COL3A1, Collagen alpha 1(III) chain, Collagen Type III, Collagen Type III (N-terminal), collagen, type III, alpha 1, EDS4A, FLJ34534

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Galerie de données de validation

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  IHC staining of human colon using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80009-1-RR (Collagen Type III (N-terminal) antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80009-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80009-1-RR (COL3A1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 80009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human colon using 80009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (80009-1-RR, Clone: 2O1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de côlon humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80009-1-RR cible Collagen Type III (N-terminal) dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Collagen Type III (N-terminal) Protéine recombinante Ag18658
• Nom complet: collagen, type III, alpha 1
• Masse moléculaire calculée: 1466 aa, 139 kDa
• Numéro d’acquisition GenBank: BC028178
• Symbole du gène: COL3A1
• Identification du gène (NCBI): 1281
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.