Anticorps Polyclonal de lapin anti-COL4A3BP

COL4A3BP Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15191-1-AP

Synonymes

Ceramide transfer protein, CERT, CERTL, COL4A3BP, GPBP, hCERT, STARD11

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Galerie de données de validation

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  WB analysis using 15191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15191-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15191-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 15191-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15191-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15191-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse brain using 15191-1-AP

  IP Result of anti-COL4A3BP (IP:15191-1-AP, 4ug; Detection:15191-1-AP 1:300) with mouse brain tissue lysate 3680ug.

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  IHC staining of human pancreas using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 15191-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu pancréatique de souris, tissu pancréatique humain, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

15191-1-AP cible COL4A3BP dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COL4A3BP Protéine recombinante Ag7277
• Nom complet: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 80-89 kDa
• Numéro d’acquisition GenBank: BC000102
• Symbole du gène: COL4A3BP
• Identification du gène (NCBI): 10087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COL4A3BP(Collagen type IV alpha-3-binding protein) is also named as CERT, STARD11. CERT extracts ceramide from the ER and carries it to the Golgi apparatus in a non-vesicular manner and that a particularly efficient cycle of CERT movement for trafficking of ceramide may proceed at membrane contact sites between the ER and the Golgi apparatus(PMID:17314061). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	Acta Biochim Biophys Sin (Shanghai); Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress-autophagy axis and improves myocardial reconstruction after acute myocardial infarction.; Authors - Yaling Li
mouse	WB	Ren Fail; LncTUG1 ameliorates renal tubular fibrosis in experimental diabetic nephropathy through the miR-145-5p/dual-specificity phosphatase 6 axis; Authors - Taoxia Wang
human	WB	Cell Rep; Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells; Authors - Yuuki Obata ; KD Validated
human,mouse	WB	Arch Biochem Biophys; The ceramide transport protein CERT is involved in alkylacylglycerol transfer from the ER to the Golgi for the biosynthesis of ether phospholipid; Authors - Yasuhiro Horibata
human,mouse	WB	Oncogenesis; Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein; Authors - Xin Tracy Liu