Anticorps Polyclonal de lapin anti-COL4A4

COL4A4 Polyclonal Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 19674-1-AP

Synonymes

COL4, Collagen Type IV, COL4A 4, collagen, type IV, alpha 4

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Galerie de données de validation

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  IF Staining of HeLa using 19674-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19674-1-AP (Collagen Type IV antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

19674-1-AP cible COL4A4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: collagen, type IV, alpha 4
• Masse moléculaire calculée: 164 kDa
• Numéro d’acquisition GenBank: NM_000092
• Symbole du gène: COL4A4
• Identification du gène (NCBI): 1286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COL4A4 belongs to the type IV collagen family. COL4A4 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). Defects in COL4A4 are a cause of benign familial hematuria (BFH). This antibody is specific to COL4A4.

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; In silico prediction and experimental validation to reveal the protective mechanism of Puerarin against excessive extracellular matrix accumulation through inhibiting ferroptosis in diabetic nephropathy; Authors - Biyu Hou
	WB	PLoS Negl Trop Dis; Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach; Authors - Yajie Yuan
rat	WB	Eur J Pharmacol; Sirt1 activation prevents anti-Thy 1.1 mesangial proliferative glomerulonephritis in the rat through the Nrf2/ARE pathway.; Authors - Kaipeng Huang
rat	IF	J Chem Neuroanat; Berberine protects against chronic cerebral hypoperfusion-induced cognitive impairment and hippocampal damage via regulation of the ERK/Nrf2 pathway.; Authors - Ningqun Wang
duck	WB	Poult Sci; Co-exposure of polyvinyl chloride microplastics with cadmium promotes nonalcoholic fatty liver disease in female ducks through oxidative stress and glycolipid accumulation; Authors - Yan Chen
human	IF	Cell; Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma; Authors - Abhirami Visvanathan

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