Anticorps Polyclonal de lapin anti-COL6A1

• Phare
• Validé par KD/KO

COL6A1 Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(38)

Avis (1)

N° de cat : 17023-1-AP

Synonymes

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

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Galerie de données de validation

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  WB analysis of HEK-293T using 17023-1-AP

  WB result of Collagen Type VI antibody (17023-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type VI transfected HEK-293T cells.

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  WB analysis using 17023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17023-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17023-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17023-1-AP

  IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.

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  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle tissue using 17023-1-AP

  IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293T, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu de cancer de la peau humain, tissu de cirrhose hépatique humain, tissu hépatique de souris, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon humain, tissu de muscle squelettique de souris
• Résultats positifs en IF: tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 9 publications below
• IF: See 10 publications below

Informations sur le produit

17023-1-AP cible COL6A1 dans les applications de WB, IHC, IF, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COL6A1 Protéine recombinante Ag10288
• Nom complet: collagen, type VI, alpha 1
• Masse moléculaire calculée: 1028 aa, 109 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC052575
• Symbole du gène: COL6A1
• Identification du gène (NCBI): 1291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Publications

Species	Application	Title
mouse,rat	IF	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
zebrafish	WB,IF	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
human,mouse	WB,IF	Cell Rep; The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis; Authors - Jingwei Jiang
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB,IHC	Oncogene; Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).; Authors - I García-Palmero
human	WB,IHC	Cancer Gene Ther; Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma; Authors - Zhuowei Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Malak (Verified Customer) (02-24-2021)

It works well in the western blot

• Applications: Western Blot

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