Anticorps Recombinant de lapin anti-COL6A3

COL6A3 Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230540D3

N° de cat : 83010-3-PBS

Synonymes

COL6A3-Specific, 230540D3, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

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Galerie de données de validation

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  FC experiment of HeLa using 83010-3-RR (same clone as 83010-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Col6a3-Specific Recombinant Antibody (83010-3-RR, Clone:230540D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83010-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83010-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83010-3-RR against Human COL6A3-Specific were performed. The affinity constant is 1.71 nM.

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Informations sur le produit

83010-3-PBS cible COL6A3 dans les applications de FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COL6A3 Protéine recombinante Ag34752
• Nom complet: collagen, type VI, alpha 3
• Masse moléculaire calculée: 344 kDa
• Poids moléculaire observé: 343 kDa
• Numéro d’acquisition GenBank: NM_004369
• Symbole du gène: COL6A3
• Identification du gène (NCBI): 1293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

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