Anticorps Polyclonal de lapin anti-COL8A1

• Phare
• Validé par KD/KO

COL8A1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 17251-1-AP

Synonymes

COL8, C3orf7, Collagen alpha-1(VIII) chain, Collagen Type VIII

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Galerie de données de validation

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  WB analysis using 17251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17251-1-AP

  IP result of anti-Collagen Type VIII (IP:17251-1-AP, 4ug; Detection:17251-1-AP 1:500) with HepG2 cells lysate 1800ug.

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  IF Staining of HepG2 using 17251-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 17251-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COL8A1 antibody (17251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules COLO 320, cellules HeLa, cellules HepG2, tissu rénal de rat, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

17251-1-AP cible COL8A1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COL8A1 Protéine recombinante Ag10607
• Nom complet: collagen, type VIII, alpha 1
• Masse moléculaire calculée: 744 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC013581
• Symbole du gène: COL8A1
• Identification du gène (NCBI): 1295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Sci China Life Sci; Identification of LRRC46 as a novel candidate gene for high myopia; Authors - Lingxi Jiang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
rat	IF	Front Cell Dev Biol; Platelet-Derived Extracellular Vesicles Increase Col8a1 Secretion and Vascular Stiffness in Intimal Injury.; Authors - Han Bao ; KD Validated
mouse	WB	FASEB J; Silencing of METTL3 attenuates cardiac fibrosis induced by myocardial infarction via inhibiting the activation of cardiac fibroblasts.; Authors - Tingting Li
rat	WB	Biomed Pharmacother; Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity.; Authors - Ke Feng
mouse	WB,IF	Dis Model Mech; Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis.; Authors - Li-Fong Seet

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (11-11-2023)

This antibody worked well for mouse eye cryosections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Eye

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