Anticorps Polyclonal de lapin anti-COLEC11

COLEC11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 15269-1-AP

Synonymes

CL K1, CL K1 I, CL K1 II, CL K1 IIa, CL K1 IIb, CLK1, COLEC11, Collectin 11, Collectin kidney protein 1, collectin sub family member 11

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Galerie de données de validation

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  WB analysis of mouse liver using 15269-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 15269-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15269-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15269-1-AP

  IP Result of anti-COLEC11 (IP:15269-1-AP, 4ug; Detection:15269-1-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IHC staining of mouse liver using 15269-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15269-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of ATCD5 cells using 15269-1-AP

  IF result (confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, plasma humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules ATCD5

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

15269-1-AP cible COLEC11 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COLEC11 Protéine recombinante Ag7374
• Nom complet: collectin sub-family member 11
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000078
• Symbole du gène: COLEC11
• Identification du gène (NCBI): 78989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)

Publications

Species	Application	Title
mouse	IHC,IF	Nat Genet; Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.; Authors - Rooryck Caroline C
human	WB,IF	Clin J Am Soc Nephrol; Collectin11 and Complement Activation in IgA Nephropathy.; Authors - Min Wei
mouse	WB	Front Immunol; Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).; Authors - Rafael Bayarri-Olmos
human	WB	J Immunol; Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1.; Authors - Anne Rosbjerg
human	FC	J Innate Immun; Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex.; Authors - Ma Ying Jie YJ
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (08-28-2019)

Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human PTECs

FH

mark (Verified Customer) (08-13-2019)

Consitent fro WB but binds non CL-11 in cell lysatesFor immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for Immuno
• Cell Tissue Type: mouse cell culture, mouse kidney, mouse embryos, human kidney

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