Anticorps Monoclonal anti-COMMD1

COMMD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B10E10

N° de cat : 67016-1-PBS

Synonymes

C2orf5, COMMD1, MURR1, Protein Murr1

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Galerie de données de validation

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  WB analysis using 67016-1-Ig (same clone as 67016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67016-1-Ig (COMMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67016-1-Ig (COMMD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67016-1-Ig (same clone as 67016-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COMMD1 antibody (67016-1-Ig, Clone: 3B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67016-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67016-1-PBS cible COMMD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COMMD1 Protéine recombinante Ag28596
• Nom complet: copper metabolism (Murr1) domain containing 1
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC022046
• Symbole du gène: COMMD1
• Identification du gène (NCBI): 150684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

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