Anticorps Polyclonal de lapin anti-COMP

COMP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 28369-1-AP

Synonymes

TSP5, Cartilage oligomeric matrix protein, PSACH, Thrombospondin-5

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Galerie de données de validation

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  WB analysis using 28369-1-AP

  Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 28369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28369-1-AP (COMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28369-1-AP (COMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28369-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (28369-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HepG2 using 28369-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug COMP Polyclonal antibody (28369-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cartilagineux de porc
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28369-1-AP cible COMP dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COMP Protéine recombinante Ag28749
• Nom complet: cartilage oligomeric matrix protein
• Masse moléculaire calculée: 757 aa, 83 kDa
• Poids moléculaire observé: 80-110 kDa
• Numéro d’acquisition GenBank: BC033676
• Symbole du gène: COMP
• Identification du gène (NCBI): 1311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others.

Publications

Species	Application	Title
mouse,human	WB	Int J Chron Obstruct Pulmon Dis; Identification of Hub Genes Associated with COPD Through Integrated Bioinformatics Analysis.; Authors - Lin Chen
human	WB	Reprod Sci; Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis; Authors - Jiazhen Huang
human	IHC	Front Mol Biosci; Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation; Authors - Yueqing Gong
mouse	IF	Eur J Pharmacol; Endonuclear Circ-calm4 Regulates Ferroptosis via a circR-Loop of the COMP Gene in Pulmonary Artery Smooth Muscle Cells; Authors - Aijing Liu

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