Anticorps Monoclonal anti-COMP

COMP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7A6

N° de cat : 66793-1-PBS

Synonymes

TSP5, 1D7A6, Cartilage oligomeric matrix protein, PSACH, Thrombospondin-5

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Galerie de données de validation

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  WB analysis of pig cartilage using 66793-1-Ig (same clone as 66793-1-PBS)

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66793-1-Ig (same clone as 66793-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 66793-1-Ig (same clone as 66793-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66793-1-PBS cible COMP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COMP Protéine recombinante Ag4575
• Nom complet: cartilage oligomeric matrix protein
• Masse moléculaire calculée: 757 aa, 83 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC033676
• Symbole du gène: COMP
• Identification du gène (NCBI): 1311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.

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