Anticorps Monoclonal anti-COMT

COMT Monoclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1A4

N° de cat : 68181-1-PBS

Synonymes

catechol O methyltransferase, COMT

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Galerie de données de validation

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  WB analysis using 68181-1-Ig (same clone as 68181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68181-1-Ig (COMT antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 68181-1-Ig (same clone as 68181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (68181-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68181-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68181-1-PBS cible COMT dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COMT Protéine recombinante Ag6502
• Nom complet: catechol-O-methyltransferase
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC000419
• Symbole du gène: COMT
• Identification du gène (NCBI): 1312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

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