Anticorps Recombinant de lapin anti-COPA

COPA Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230361B1

N° de cat : 83089-2-PBS

Synonymes

HEP COP, FLJ26320, Coatomer subunit alpha, Alpha COP, Alpha coat protein

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Galerie de données de validation

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  WB analysis using 83089-2-RR (same clone as 83089-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83089-2-RR (COPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83089-2-RR (same clone as 83089-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83089-2-RR (COPA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using COPA antibody (83089-2-RR, Clone: 230361B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83089-2-RR (same clone as 83089-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COPA Recombinant antibody (83089-2-RR, Clone:230361B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83089-2-PBS in a different storage buffer formulation.

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Informations sur le produit

83089-2-PBS cible COPA dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COPA Protéine recombinante Ag34207
• Nom complet: coatomer protein complex, subunit alpha
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_004371
• Symbole du gène: COPA
• Identification du gène (NCBI): 1314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.

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