Anticorps Polyclonal de lapin anti-CSN2

• Phare
• Validé par KD/KO

CSN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10969-2-AP

Synonymes

ALIEN, Alien homolog, COPS 2, COPS2, COPS2/TRIP15, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

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Galerie de données de validation

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  WB analysis using 10969-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10969-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10969-2-AP

  WB result of CSN2 antibody (10969-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSN2 transfected HEK-293 cells.

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  WB analysis of mouse brain using 10969-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10969-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10969-2-AP

  IP Result of anti-CSN2 (IP:10969-2-AP, 3ug; Detection:10969-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

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  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10969-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSN2 antibody (10969-2-AP) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HEK-293T, cellules NIH/3T3, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10969-2-AP cible CSN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSN2 Protéine recombinante Ag1415
• Nom complet: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC012629
• Symbole du gène: COPS2
• Identification du gène (NCBI): 9318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.

Publications

Species	Application	Title
human	WB	Mol Cell; Glucose-induced CRL4COP1-p53 axis amplifies glycometabolism to drive tumorigenesis; Authors - Yang Su
mouse	WB	Nat Commun; IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.; Authors - Hong Lin ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.; Authors - Feng Rao
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.; Authors - Paul C Scherer
human	WB	Proc Natl Acad Sci U S A; Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.; Authors - Hong Lin
human	WB	iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Resha (Verified Customer) (11-01-2023)

In 20ug of total protein, 1:1000 dilution of anti-COPS2 antibody gave clear band of 52kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells