Anticorps Recombinant de lapin anti-COPS3

• Phare
• Validé par KD/KO

COPS3 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240918E3

N° de cat : 83822-2-PBS

Synonymes

240918E3, COP 9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3

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Galerie de données de validation

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  WB analysis using 83822-2-RR (same clone as 83822-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83822-2-RR (COPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83822-2-RR (same clone as 83822-2-PBS)

  WB result of COPS3 antibody (83822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells. This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83822-2-RR (same clone as 83822-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83822-2-RR (COPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00797-1

  Cytometric bead array standard curve of MP00797-1, COPS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83822-4-PBS. Detection antibody: 83822-2-PBS. Standard: Ag32825. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83822-2-RR against Human COPS3 were performed. The affinity constant is 1.29 nM.

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Informations sur le produit

83822-2-PBS cible COPS3 dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COPS3 Protéine recombinante Ag32825
• Nom complet: COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC001891
• Symbole du gène: COPS3
• Identification du gène (NCBI): 8533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

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