Anticorps Polyclonal de lapin anti-COPS4

COPS4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10464-1-PBS

Synonymes

COPS, COPS 4, CSN4, JAB1 containing signalosome subunit 4, JAB1-containing signalosome subunit 4

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Galerie de données de validation

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  WB analysis of mouse brain using 10464-1-AP (same clone as 10464-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10464-1-AP (same clone as 10464-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 10464-1-AP (same clone as 10464-1-PBS)

  IP result of anti-COPS4 (IP:10464-1-AP, 4ug; Detection:10464-1-AP 1:300) with PC-3 cells lysate 1800ug. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10464-1-AP (same clone as 10464-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 10464-1-AP (same clone as 10464-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10464-1-PBS cible COPS4 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COPS4 Protéine recombinante Ag0735
• Nom complet: COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 40 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC004302
• Symbole du gène: COPS4
• Identification du gène (NCBI): 51138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).

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