Anticorps Polyclonal de lapin anti-COPS6

• Phare
• Validé par KD/KO

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, singe, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10256-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10256-1-PBS cible COPS6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, singe, souris

• Réactivité: Humain, porc, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COPS6 Protéine recombinante Ag0331
• Nom complet: COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
• Masse moléculaire calculée: 327 aa, 36 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC002520
• Symbole du gène: COPS6
• Identification du gène (NCBI): 10980
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

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