Anticorps Polyclonal de lapin anti-COPZ1

• Phare
• Validé par KD/KO

COPZ1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20440-1-AP

Synonymes

CGI 120, CGI-120, Coatomer subunit zeta 1, Coatomer subunit zeta-1, COPZ

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Galerie de données de validation

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  WB analysis using 20440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20440-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 20440-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20440-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 20440-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using COPZ1 antibody (20440-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules COS-7, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

20440-1-AP cible COPZ1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COPZ1 Protéine recombinante Ag14234
• Nom complet: coatomer protein complex, subunit zeta 1
• Masse moléculaire calculée: 177 aa, 20 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC002849
• Symbole du gène: COPZ1
• Identification du gène (NCBI): 22818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Blood; A new severe congenital neutropenia syndrome associated with autosomal recessive COPZ1 mutations; Authors - Natalia Borbaran Bravo
human	WB	MedComm (2020); Chemoproteomics enables identification of coatomer subunit zeta-1 targeted by a small molecule for enterovirus A71 inhibition; Authors - Xiaoyong Li
mouse	WB	Biochim Biophys Acta Gen Subj; COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma; Authors - Anbang Wu ; KD Validated
human	WB	Genes (Basel); A Comprehensive Pan-Cancer Analysis of the Regulation and Prognostic Effect of Coat Complex Subunit Zeta 1; Authors - Ye Hong ; KD Validated

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