Anticorps Polyclonal de lapin anti-COQ10A

COQ10A Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 17812-1-AP

Synonymes

Coenzyme Q-binding protein COQ10 homolog A, mitochondrial

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Galerie de données de validation

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  WB analysis of human heart using 17812-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17812-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17812-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17812-1-AP (COQ10A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17812-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17812-1-AP (COQ10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

17812-1-AP cible COQ10A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COQ10A Protéine recombinante Ag12126
• Nom complet: coenzyme Q10 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC073923
• Symbole du gène: COQ10A
• Identification du gène (NCBI): 93058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
Human	WB	Front Oncol; Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells.; Authors - Chen Sun
human	WB,IHC	Front Pharmacol; Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro.; Authors - Meng-Yun Zhao
human	WB	Mol Med Rep; Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.; Authors - Bingju Yan
rat	WB	Mol Med Rep; UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.; Authors - Bingju Yan
human	WB	J Lipid Res; Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function.; Authors - Hui S Tsui

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