Anticorps Polyclonal de lapin anti-COQ7

• Phare
• Validé par KD/KO

COQ7 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 15083-1-AP

Synonymes

CAT5, CLK 1, CLK1, COQ7, Timing protein clk 1 homolog

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Galerie de données de validation

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  WB analysis of mouse liver using 15083-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15083-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15083-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15083-1-AP

  IP Result of anti-COQ7 (IP:15083-1-AP, 4ug; Detection:15083-1-AP 1:500) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver cancer using 15083-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15083-1-AP (COQ7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 15083-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15083-1-AP (COQ7 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cardiaque de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IF: See 1 publications below

Informations sur le produit

15083-1-AP cible COQ7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COQ7 Protéine recombinante Ag7161
• Nom complet: coenzyme Q7 homolog, ubiquinone (yeast)
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003185
• Symbole du gène: COQ7
• Identification du gène (NCBI): 10229
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COQ7(Ubiquinone biosynthesis protein COQ7 homolog) catalyzes the production of coenzyme Q (CoQ) in mitochondria. The predicted protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and 3 N-myristoylation sites. This protein has 2 isoforms produced by alternative splicing with MW 20-24 kDa.

Publications

Species	Application	Title
human	WB, IF	Nat Cell Biol; A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.; Authors - Richard M Monaghan
human	WB	J Clin Invest; ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption.; Authors - Ashraf Shazia S ; KD Validated
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
mouse	WB	Acta Pharmacol Sin; Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice.; Authors - Peng-Ju Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ying (Verified Customer) (04-26-2021)

It's super clean and works great.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse and human fibroblasts, mouse tissues and human muscle biopsy samples