Anticorps Polyclonal de lapin anti-CORO1C

• Phare
• Validé par KD/KO

CORO1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(14)

Avis (1)

N° de cat : 14749-1-AP

Synonymes

CRNN4, CRN2, Coronin-3, Coronin-1C, Coronin 3

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Galerie de données de validation

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  WB analysis of mouse heart using 14749-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14749-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14749-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14749-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 14749-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 14749-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14749-1-AP

  IP result of anti-CORO1C (IP:14749-1-AP, 4ug; Detection:14749-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IP experiment of mouse heart using 14749-1-AP

  IP result of anti-CORO1C (IP:14749-1-AP, 4ug; Detection:14749-1-AP 1:3000) with mouse heart tissue lysate 1680 ug.

• 

  IHC staining of human cervical cancer using 14749-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14749-1-AP (CORO1C antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14749-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14749-1-AP (CORO1C antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 14749-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14749-1-AP (CORO1C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 14749-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CORO1C antibody (14749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, cellules SGC-7901, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, human normal colon; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14749-1-AP cible CORO1C dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CORO1C Protéine recombinante Ag6488
• Nom complet: coronin, actin binding protein, 1C
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC002342
• Symbole du gène: CORO1C
• Identification du gène (NCBI): 23603
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CORO1C also known as HCRNN4, belongs to the WD repeat coronin family. CORO1C contains 5 N-terminal trp-asp (WD) repeats and a C-terminal coiled-coil motif. It was reported to regulate actin‐dependent processes by assembling F-actin. CORO1C promoted the invasion of breast cancer and glioma cells by specifically promoting the formation of invadopodia(PMID: 30974047).

Publications

Species	Application	Title
mouse	IF	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
monkey	WB	Cell; A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.; Authors - Melissa J Hoyer ; KD Validated
human	WB	J Cell Biol; Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission.; Authors - Jonathan F Striepen
mouse	WB	Hum Mol Genet; Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice.; Authors - Laura Pellegrini
human	WB, IHC	Front Mol Biosci; CORO1C is Associated With Poor Prognosis and Promotes Metastasis Through PI3K/AKT Pathway in Colorectal Cancer.; Authors - Zongxia Wang
human	WB	Genes (Basel); Coronin 1C, Regulated by Multiple microRNAs, Facilitates Cancer Cell Aggressiveness in Pancreatic Ductal Adenocarcinoma; Authors - Kosuke Fukuda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wen (Verified Customer) (12-22-2019)

Work well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse haematopoietic cells

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