Anticorps Polyclonal de lapin anti-COSMC

• Phare
• Validé par KD/KO

COSMC Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19254-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)

  WB result of COSMC antibody (19254-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COSMC transfected HepG2 cells. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 19254-1-AP (same clone as 19254-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19254-1-PBS cible COSMC dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COSMC Protéine recombinante Ag6899
• Nom complet: C1GALT1-specific chaperone 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-37 kDa
• Numéro d’acquisition GenBank: BC050441
• Symbole du gène: COSMC
• Identification du gène (NCBI): 29071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.

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