Anticorps Monoclonal anti-COTL1

• Phare
• Validé par KD/KO

COTL1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A3D9

N° de cat : 60237-1-PBS

Synonymes

CLP, Coactosin like protein, COTL1

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Galerie de données de validation

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  WB analysis of A431 using 60237-1-Ig (same clone as 60237-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60237-1-Ig (same clone as 60237-1-PBS)

  WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60237-1-Ig (same clone as 60237-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 60237-1-Ig (same clone as 60237-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60237-1-PBS cible COTL1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COTL1 Protéine recombinante Ag1230
• Nom complet: coactosin-like 1 (Dictyostelium)
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 14-16 kDa
• Numéro d’acquisition GenBank: BC010884
• Symbole du gène: COTL1
• Identification du gène (NCBI): 23406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

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