Anticorps Monoclonal anti-SARS-CoV-2 S protein (RBD, 319-541 aa)
SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal Antibody for WB, Neutralization, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Virus et plus (2)
Applications
WB, IF, Neutralization, ELISA
Conjugaison
Non conjugué
CloneNo.
1H3E9
N° de cat : 67758-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | Ag30688, |
Résultats positifs en Neutralization | Protéine recombinante, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
NEUTRALIZATION | NEUTRALIZATION : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 9 publications below |
IF | See 1 publications below |
Informations sur le produit
67758-1-Ig cible SARS-CoV-2 S protein (RBD, 319-541 aa) dans les applications de WB, IF, Neutralization, ELISA et montre une réactivité avec des échantillons Virus
Réactivité | Virus |
Réactivité citée | Humain, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | SARS-CoV-2 S protein (RBD, 319-541 aa) Protéine recombinante Ag30688 |
Nom complet | SARS-CoV-2 Spike Protein |
Masse moléculaire calculée | 141 kDa |
Numéro d’acquisition GenBank | NC_045512 |
Symbole du gène | SARS-COV-2 |
Identification du gène (NCBI) | 43740568 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
Lyophilized format of this product is available.
Protocole
Product Specific Protocols | |
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WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants | ||
J Med Virol A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector | ||
bioRxiv Novel sensor-integrated proteome on chip (SPOC) platform with thousands of folded proteins on a 1.5 sq-cm biosensor chip to enable high-throughput real-time label-free screening for kinetic analysis | ||
Virol J LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19 | ||
Int J Mol Sci SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia |