Anticorps Recombinant de lapin anti-COX-1/Cyclooxygenase-1

COX-1/Cyclooxygenase-1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241373C10

N° de cat : 84151-4-PBS

Synonymes

PGH1, PTGS1, 241373C10, COX1, Cyclooxygenase-1

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Galerie de données de validation

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  WB analysis using 84151-4-RR (same clone as 84151-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 84151-4-RR (same clone as 84151-4-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 84151-4-RR (same clone as 84151-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 84151-4-RR (same clone as 84151-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84151-4-RR (same clone as 84151-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using COX-1/Cyclooxygenase-1 antibody (84151-4-RR, Clone: 241373C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84151-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84151-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84151-4-PBS cible COX-1/Cyclooxygenase-1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COX-1/Cyclooxygenase-1 Protéine recombinante Ag4055
• Nom complet: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 60-72 kDa
• Numéro d’acquisition GenBank: BC029840
• Symbole du gène: PTGS1
• Identification du gène (NCBI): 5742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

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