Anticorps Recombinant de lapin anti-COX-1/Cyclooxygenase-1

COX-1/Cyclooxygenase-1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241373C10

N° de cat : 84151-4-RR

Synonymes

PGH1, PTGS1, 241373C10, COX1, Cyclooxygenase-1

---

Galerie de données de validation

• 

  WB analysis using 84151-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 84151-4-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse stomach using 84151-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 84151-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84151-4-RR (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 84151-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using COX-1/Cyclooxygenase-1 antibody (84151-4-RR, Clone: 241373C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84151-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84151-4-RR against Human COX-1/Cyclooxygenase-1 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules C2C12, cellules NIH/3T3, cellules THP-1
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84151-4-RR cible COX-1/Cyclooxygenase-1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COX-1/Cyclooxygenase-1 Protéine recombinante Ag4055
• Nom complet: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 60-72 kDa
• Numéro d’acquisition GenBank: BC029840
• Symbole du gène: PTGS1
• Identification du gène (NCBI): 5742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

{{ptg:RelatedPrimaryAntibodies}}