Anticorps Monoclonal anti-COX2/ Cyclooxygenase 2/ PTGS2

• Phare
• Validé par KD/KO

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(212)

CloneNo.

3G2B9

N° de cat : 66351-1-Ig

Synonymes

COX2, PTGS2, 3G2B9, EC:1.14.99.1, Cyclooxygenase-2

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Galerie de données de validation

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  WB analysis using 66351-1-Ig

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66351-1-Ig

  Untreated and LPS-treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66351-1-Ig

  Untreated and LPS-treated Hela cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HeLa traitées au LPS, cellules RAW 264.7 traitées au LPS
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pulmonaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 180 publications below
• IHC: See 28 publications below
• IF: See 23 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66351-1-Ig cible COX2/ Cyclooxygenase 2/ PTGS2 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COX2/ Cyclooxygenase 2/ PTGS2 Protéine recombinante Ag24721
• Nom complet: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 604 aa, 68 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC013734
• Symbole du gène: COX2/PTGS2
• Identification du gène (NCBI): 5743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.

1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).

Publications

Species	Application	Title
human	WB	Cell Metab; Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.; Authors - David Pacheu-Grau
	IHC	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
mouse	WB	Adv Sci (Weinh); Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis; Authors - Fangyi Xu
mouse	WB	J Adv Res; Exploiting metabolic vulnerabilities through synergistic ferroptosis and disulfidptosis for breast cancer therapy; Authors - Yu Liang
human	WB	Redox Biol; The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3; Authors - Jian Zhu
mouse	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan

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