Anticorps Polyclonal de lapin anti-COX5A

COX5A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 11448-1-AP

Synonymes

COX, COX VA, COX5A, VA

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Galerie de données de validation

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  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11448-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11448-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 11448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11448-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11448-1-AP (COX5A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11448-1-AP

  Immunofluorescent analysis of HeLa cells using 11448-1-AP (COX5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules L02, tissu cérébral de rat, tissu hépatique humain
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 3 publications below

Informations sur le produit

11448-1-AP cible COX5A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, singe, souris, Chimpanzé
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX5A Protéine recombinante Ag2005
• Nom complet: cytochrome c oxidase subunit Va
• Masse moléculaire calculée: 150 aa, 17 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC024240
• Symbole du gène: COX5A
• Identification du gène (NCBI): 9377
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Cancer Res; CK1δ/CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma; Authors - Karen L Burger
mouse	WB	Dev Cell; Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain.; Authors - Shefali Krishna
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
pig	IHC	Int J Mol Sci; Relation of Plasma Catecholamine Concentrations and Myocardial Mitochondrial Respiratory Activity in Anesthetized and Mechanically Ventilated, Cardiovascular Healthy Swine; Authors - Nadja Abele
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang