Anticorps Polyclonal de lapin anti-COX5B

• Phare
• Validé par KD/KO

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 11418-2-AP

Synonymes

COXVB

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Galerie de données de validation

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  WB analysis of HeLa using 11418-2-AP

  WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells.

• 

  WB analysis using 11418-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11418-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11418-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11418-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX5B antibody (11418-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 4 publications below

Informations sur le produit

11418-2-AP cible COX5B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX5B Protéine recombinante Ag1986
• Nom complet: cytochrome c oxidase subunit Vb
• Masse moléculaire calculée: 129 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC006229
• Symbole du gène: COX5B
• Identification du gène (NCBI): 1329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

Publications

Species	Application	Title
human	WB	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy
mouse	WB	Nature; Mechanism of super-assembly of respiratory complexes III and IV.; Authors - Sara Cogliati
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein ; KD Validated
mouse	WB	Nat Immunol; Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense.; Authors - Johan Garaude
human	WB	Cell Metab; Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle.; Authors - Chiara Greggio
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Süleyman (Verified Customer) (02-07-2022)

HeLa cell line is used and even though it has some unspecific bands but it is very specific and the unspecific bands are very distant. Recommended!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

COX5B Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:11418-2-AP)

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