Anticorps Polyclonal de lapin anti-COX5B

• Phare
• Validé par KD/KO

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11418-2-PBS

Synonymes

COXVB

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Galerie de données de validation

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  WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

  WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11418-2-AP (same clone as 11418-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX5B antibody (11418-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11418-2-PBS cible COX5B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX5B Protéine recombinante Ag1986
• Nom complet: cytochrome c oxidase subunit Vb
• Masse moléculaire calculée: 129 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC006229
• Symbole du gène: COX5B
• Identification du gène (NCBI): 1329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

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