Anticorps Polyclonal de lapin anti-COX7A1

COX7A1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11413-1-AP

Synonymes

COX7A, COX7A1, COX7AH, COX7AM

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Galerie de données de validation

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  WB analysis of mouse heart using 11413-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11413-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11413-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11413-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11413-1-AP (COX7A1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11413-1-AP cible COX7A1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX7A1 Protéine recombinante Ag1981
• Nom complet: cytochrome c oxidase subunit VIIa polypeptide 1 (muscle)
• Masse moléculaire calculée: 79 aa, 9 kDa
• Poids moléculaire observé: 7-9 kDa
• Numéro d’acquisition GenBank: BC002757
• Symbole du gène: COX7A1
• Identification du gène (NCBI): 1346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c oxidase(COX) catalyzes the transfer of electrons from reduced cytochrome c to molecular oxygen. Subunit VIIa of mammalian COX exists in at least 2 isoforms, liver and muscle. COX7A1(Cytochrome c oxidase subunit 7A1, mitochondrial) is also named as COX7AH, cytochrome c oxidase subunit VIIa-H, cytochrome c oxidase subunit VIIa-M and is the muscle isoform of COX subunit VIIa. Northern-blot analysis of primate tissues demonstrated that COXVIIa-M mRNA is present only in muscle tissues(PMID:1327965).

Publications

Species	Application	Title
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti
human	WB	Lab Invest; Overexpression of COX7A1 Promotes the Resistance of Gastric Cancer to Oxaliplatin and Weakens the Efficacy of Immunotherapy; Authors - Si-Yu Wang
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
human	WB, IF	Int J Biochem Cell Biol; Investigating the role of the physiological isoform switch of cytochrome c oxidase subunits in reversible mitochondrial disease.; Authors - Veronika Boczonadi
mouse	WB	Biochim Biophys Acta Mol Cell Res; Blockade of angiotensin II modulates insulin-like growth factor 1-mediated skeletal muscle homeostasis in experimental steatohepatitis; Authors - Misako Tanaka
human	WB	Cell Death Dis; BNIP3-mediated mitophagy boosts the competitive growth of Lenvatinib-resistant cells via energy metabolism reprogramming in HCC; Authors - Sikai Wang

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