Anticorps Polyclonal de lapin anti-COX7A2L

• Phare
• Validé par KD/KO

COX7A2L Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 11416-1-AP

Synonymes

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81

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Galerie de données de validation

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  WB analysis using 11416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11416-1-AP (COX7A2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11416-1-AP cible COX7A2L dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX7A2L Protéine recombinante Ag1980
• Nom complet: cytochrome c oxidase subunit VIIa polypeptide 2 like
• Masse moléculaire calculée: 114 aa, 13 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC007095
• Symbole du gène: COX7A2L
• Identification du gène (NCBI): 9167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Publications

Species	Application	Title
mouse	WB	Science; Supercomplex assembly determines electron flux in the mitochondrial electron transport chain.; Authors - Esther Lapuente-Brun
human	WB	EMBO J; Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.; Authors - Teresa Lobo-Jarne
human	WB	Cell Rep; Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.; Authors - Teresa Lobo-Jarne ; KO Validated
		Free Radic Biol Med; Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.; Authors - I Ramírez-Camacho
human	WB	Cell Rep; COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.; Authors - Rafael Pérez-Pérez
human	WB,IHC,IF	J Pathol; Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.; Authors - Aichler Michaela M ; KD Validated