Anticorps Monoclonal anti-COXIV

COXIV Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A7B2

N° de cat : 66110-1-PBS

Synonymes

COX4I1, 2A7B2, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

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Galerie de données de validation

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  WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  WB analysis using 66110-1-Ig (same clone as 66110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66110-1-Ig (same clone as 66110-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66110-1-Ig (COXIV antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66110-1-Ig (COXIV antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66110-1-Ig (same clone as 66110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66110-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66110-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50551-3

  Cytometric bead array standard curve of MP50551-3, COXIV Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66110-1-PBS. Detection antibody: 60251-4-PBS. Standard:Ag1640. Range: 1.563-200 ng/mL.

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Informations sur le produit

66110-1-PBS cible COXIV dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: COXIV Protéine recombinante Ag20551
• Nom complet: cytochrome c oxidase subunit IV isoform 1
• Masse moléculaire calculée: 19.6 kDa
• Poids moléculaire observé: 17-18 kDa
• Numéro d’acquisition GenBank: BC021236
• Symbole du gène: COX IV
• Identification du gène (NCBI): 1327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

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