Anticorps Recombinant de lapin anti-COXIV

• Phare
• Validé par KD/KO

COXIV Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3K20

N° de cat : 82916-1-PBS

Synonymes

COX4I1, 3K20, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

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Galerie de données de validation

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  WB analysis of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

  WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82916-1-RR (same clone as 82916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82916-1-RR (same clone as 82916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82916-1-RR (same clone as 82916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82916-1-RR (COXIV antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82916-1-RR (same clone as 82916-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82916-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82916-1-RR against Human COXIV were performed. The affinity constant is 9.41 nM.

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Informations sur le produit

82916-1-PBS cible COXIV dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COXIV Protéine recombinante Ag1640
• Nom complet: cytochrome c oxidase subunit IV isoform 1
• Masse moléculaire calculée: 19.6 kDa
• Poids moléculaire observé: 17-18 kDa
• Numéro d’acquisition GenBank: BC021236
• Symbole du gène: COX IV
• Identification du gène (NCBI): 1327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.

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