Anticorps Monoclonal anti-CPA1

CPA1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2H2E8

N° de cat : 68826-1-PBS

Synonymes

Carboxypeptidase A1, 2H2E8, CPA, EC:3.4.17.1

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Galerie de données de validation

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  WB analysis using 68826-1-Ig (same clone as 68826-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68826-1-PBS cible CPA1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPA1 Protéine recombinante Ag8579
• Nom complet: carboxypeptidase A1 (pancreatic)
• Masse moléculaire calculée: 419 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC005279
• Symbole du gène: Carboxypeptidase A1
• Identification du gène (NCBI): 1357
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

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