Anticorps Polyclonal de lapin anti-Carboxypeptidase A3

Carboxypeptidase A3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 16236-1-AP

Synonymes

CPA3, MC-CPA, MC CPA, Mast cell carboxypeptidase A, EC:3.4.17.1

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Galerie de données de validation

• 

  WB analysis of mouse pancreas using 16236-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16236-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'intestin grêle humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16236-1-AP cible Carboxypeptidase A3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Carboxypeptidase A3 Protéine recombinante Ag8984
• Nom complet: carboxypeptidase A3 (mast cell)
• Masse moléculaire calculée: 417 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC012613
• Symbole du gène: Carboxypeptidase A3
• Identification du gène (NCBI): 1359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Am J Pathol; Rare Pulmonary Connective Tissue Type Mast Cells Regulate Lung Endothelial Cell Angiogenesis.; Authors - Yue Ren
mouse	IF	Dev Cell; Mast cells proliferate in the peri-hippocampal space during early development and modulate local and peripheral immune cells; Authors - Alexa C Blanchard

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