Anticorps Recombinant de lapin anti-CPE

CPE Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250680F12

N° de cat : 86111-1-PBS

Synonymes

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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Galerie de données de validation

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  WB analysis using 86111-1-RR (same clone as 86111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86111-1-RR (CPE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02472-1

  Sandwich ELISA standard curve of MP02472-1, Human CPE Recombinant Matched Antibody Pair - PBS only. 86111-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2989. 86111-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL

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  Sample test of MP02472-1

  Serum of fifteen individual healthy human donors was measured. The human CPE concentration of detected samples was determined to be 5.22 ng/mL with a range of 0.76 - 15.81 ng/mL.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 86111-1-RR (same clone as 86111-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CPE antibody (86111-1-RR, Clone: 250680F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86111-1-PBS cible CPE dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CPE Protéine recombinante Eg2989
• Nom complet: carboxypeptidase E
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: NM_001873.4
• Symbole du gène: Carboxypeptidase E
• Identification du gène (NCBI): 1363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

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