Anticorps Recombinant de lapin anti-CPLX1

CPLX1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250131A9

N° de cat : 85874-1-RR

Synonymes

Complexin-1, CPLX 1, CPX I, CPX1, CPXI

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 85874-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85874-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85874-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85874-1-RR against Human CPLX1 were performed. The affinity constant is 0.717 nM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat, tissu de cervelet de rat, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85874-1-RR cible CPLX1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CPLX1 Protéine recombinante Ag0387
• Nom complet: complexin 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC002471
• Symbole du gène: CPLX1
• Identification du gène (NCBI): 10815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

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