Anticorps Polyclonal de lapin anti-CPLX2

• Phare
• Validé par KD/KO

CPLX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 18149-1-AP

Synonymes

CPX II, CPX 2, CPLX 2, Complexin-2, 921L

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Galerie de données de validation

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  WB analysis of mouse brain using 18149-1-AP

  mouse and rat brain tissue were subjected to SDS PAGE followed by western blot with 18149-1-AP (CPLX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18149-1-AP

  WB result of CPLX2 antibody (18149-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX2 transfected A549 cells.

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  IP experiment of A549 using 18149-1-AP

  IP result of anti-CPLX2 (IP:18149-1-AP, 4ug; Detection:18149-1-AP 1:1000) with A549 cells lysate 600 ug.

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  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 18149-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CPLX2 antibody (18149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cérébral de rat
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

18149-1-AP cible CPLX2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPLX2 Protéine recombinante Ag12838
• Nom complet: complexin 2
• Masse moléculaire calculée: 134 aa, 15 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC093706
• Symbole du gène: CPLX2
• Identification du gène (NCBI): 10814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Publications

Species	Application	Title
mouse	WB,IF	Acta Pharmacol Sin; Complexin 2 contributes to the protective effect of NAD+ on neuronal survival following neonatal hypoxia-ischemia; Authors - Xiao-Wen Xu
mouse	WB	Life Sci; Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction; Authors - Yu Ma
mouse	WB	Free Radic Biol Med; NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.; Authors - Wang Ziying Z ; KD Validated
mouse	WB	Behav Brain Res; Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running.; Authors - Marius Walus
human	IHC	Cancer Biomark; Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors.; Authors - Komatsu Hiroaki H
mouse	IF	bioRxiv; Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans; Authors - Xue Wen Ng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

julie (Verified Customer) (03-28-2022)

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• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: N2a cells, mouse tissue (spinal cord)

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