Anticorps Recombinant de lapin anti-CPN1

CPN1 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241481A5

N° de cat : 84218-1-PBS

Synonymes

241481A5, ACBP, Arginine carboxypeptidase, CPN, EC:3.4.17.3

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Galerie de données de validation

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  WB analysis of human plasma using 84218-1-RR (same clone as 84218-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84218-1-RR (CPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CPN1 antibody (84218-1-RR, Clone: 241481A5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01152-1

  Cytometric bead array standard curve of MP01152-1, CPN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84218-2-PBS. Detection antibody: 84218-1-PBS. Standard: Ag4035. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84218-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84218-1-PBS against Human CPN1 was performed. The affinity constant is 12.4 nM.

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Informations sur le produit

84218-1-PBS cible CPN1 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CPN1 Protéine recombinante Ag4035
• Nom complet: carboxypeptidase N, polypeptide 1
• Masse moléculaire calculée: 458 aa, 52 kDa
• Poids moléculaire observé: 40~50 kDa
• Numéro d’acquisition GenBank: BC027897
• Symbole du gène: CPN1
• Identification du gène (NCBI): 1369
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.

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