Anticorps Recombinant de lapin anti-CPS1

CPS1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241198F1

N° de cat : 84277-1-PBS

Synonymes

EC:6.3.4.16, CPSase I, Carbamoyl-phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], mitochondrial, 241198F1

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Galerie de données de validation

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  WB analysis of HeLa using 84277-1-RR (same clone as 84277-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  WB analysis using 84277-1-RR (same clone as 84277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84277-1-RR (CPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84277-1-RR (same clone as 84277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84277-1-RR (CPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84277-1-RR (same clone as 84277-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CPS1 antibody (84277-1-RR, Clone: 241198F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84277-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84277-1-PBS cible CPS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: carbamoyl-phosphate synthetase 1, mitochondrial
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 140-165 kDa
• Numéro d’acquisition GenBank: BC140943
• Symbole du gène: CPS1
• Identification du gène (NCBI): 1373
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

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