Anticorps Recombinant de lapin anti-CPSF2

CPSF2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251387H5

N° de cat : 86513-3-RR

Synonymes

CPSF 100 kDa subunit, CPSF100, KIAA1367

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Galerie de données de validation

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  WB analysis using 86513-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86513-3-RR (CPSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86513-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPSF2 antibody (86513-3-RR, Clone: 251387H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86513-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86513-3-RR against Human CPSF2 were performed. The affinity constant is 0.487 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules Ramos, cellules RAW 264.7, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86513-3-RR cible CPSF2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CPSF2 Protéine recombinante Ag11950
• Nom complet: cleavage and polyadenylation specific factor 2, 100kDa
• Masse moléculaire calculée: 782 aa, 88 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC070095
• Symbole du gène: CPSF2
• Identification du gène (NCBI): 53981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673)

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