Anticorps Polyclonal de lapin anti-CPSF6

• Phare
• Validé par KD/KO

CPSF6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 15489-1-AP

Synonymes

CFIM, CFIM68, CPSF 68 kDa subunit, CPSF6, HPBRII 4, HPBRII 7, Protein HPBRII 4/7

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Galerie de données de validation

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  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15489-1-AP

  IP Result of anti-CPSF6 (IP:15489-1-AP, 4ug; Detection:15489-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SW480 using 15489-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CPSF6 antibody (15489-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MDA-MB-231, cellules PC-3, cellules SW480
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SW480,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below

Informations sur le produit

15489-1-AP cible CPSF6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CPSF6 Protéine recombinante Ag7852
• Nom complet: cleavage and polyadenylation specific factor 6, 68kDa
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 55-68 kDa
• Numéro d’acquisition GenBank: BC000714
• Symbole du gène: CPSF6
• Identification du gène (NCBI): 11052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

Publications

Species	Application	Title
human	WB	Mol Cell; A Quantitative Genetic Interaction Map of HIV Infection.; Authors - David E Gordon
human	WB	PLoS Pathog; In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.; Authors - Matthew S Henning
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	WB	J Virol; Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.; Authors - Augustin Penda Twizerimana
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	J Virol; Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.; Authors - Akatsuki Saito ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells