Anticorps Recombinant de lapin anti-CPSF7

CPSF7 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242644E1

N° de cat : 85203-1-PBS

Synonymes

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

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Galerie de données de validation

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  WB analysis using 85203-1-RR (same clone as 85203-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01956-1

  Sandwich ELISA standard curve of MP01956-1, Human CPSF7 Recombinant Matched Antibody Pair - PBS only. 85203-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01255. 85203-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01956-1

  The mean CPSF7 concentration was determined to be 4,199.1 pg/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load and 1,800.3 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of A431 using 85203-1-RR (same clone as 85203-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85203-1-PBS cible CPSF7 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: pre-mRNA cleavage factor I, 59 kDa subunit
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_001136040
• Symbole du gène: CPSF7
• Identification du gène (NCBI): 79869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

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