Anticorps Monoclonal anti-CPT1A

• Phare
• Validé par KD/KO

CPT1A Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5D8G9

N° de cat : 66039-1-PBS

Synonymes

5D8G9, Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1

---

Galerie de données de validation

• 

  WB analysis of A549 using 66039-1-Ig (same clone as 66039-1-PBS)

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 66039-1-Ig (same clone as 66039-1-PBS)

  IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPT1A antibody (66039-1-Ig, Clone: 5D8G9 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Monoclonal antibody (66039-1-Ig, Clone:5D8G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50197-1

  Cytometric bead array standard curve of MP50197-1, CPT1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66039-1-PBS. Detection antibody: 66039-2-PBS. Standard:Ag7745. Range: 0.195-50 ng/mL.

---

Informations sur le produit

66039-1-PBS cible CPT1A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPT1A Protéine recombinante Ag7745
• Nom complet: carnitine palmitoyltransferase 1A (liver)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC000185
• Symbole du gène: CPT1A
• Identification du gène (NCBI): 1374
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

{{ptg:RelatedPrimaryAntibodies}}