Anticorps Monoclonal anti-CPT1A

CPT1A Monoclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

5D2B8

N° de cat : 66039-3-Ig

Synonymes

5D2B8, Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1

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Galerie de données de validation

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  WB analysis using 66039-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66039-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66039-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66039-3-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66039-3-Ig (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 66039-3-Ig

  IP result of anti-CPT1A (IP:66039-3-Ig, 4ug; Detection:66039-3-Ig 1:3000) with MCF-7 cells lysate 1520 ug.

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  IP experiment of HepG2 using 66039-3-Ig

  IP result of anti-CPT1A (IP:66039-3-Ig, 4ug; Detection:66039-3-Ig 1:3000) with HepG2 cells lysate 1800 ug.

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  IHC staining of mouse heart using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66039-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66039-3-Ig (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: TT cells, cellules HaCaT, cellules HeLa, cellules LNCaP, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules MCF-7, cellules HepG2
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66039-3-Ig cible CPT1A dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPT1A Protéine recombinante Ag7745
• Nom complet: carnitine palmitoyltransferase 1A (liver)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC000185
• Symbole du gène: CPT1A
• Identification du gène (NCBI): 1374
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

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