Anticorps Monoclonal anti-CPT2

CPT2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3F11C10

N° de cat : 68815-3-Ig

Synonymes

Carnitine O-palmitoyltransferase 2, mitochondrial, carnitine palmitoyltransferase 2, CPT II, CPTASE, EC:2.3.1.21

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Galerie de données de validation

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  WB analysis of rat liver using 68815-3-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68815-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68815-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68815-3-Ig cible CPT2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CPT2 Protéine recombinante Ag24833
• Nom complet: carnitine palmitoyltransferase 2
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002445
• Symbole du gène: CPT2
• Identification du gène (NCBI): 1376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

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